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p nk κb  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p nk κb
    P Nk κb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 9256 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+nk+%CE%BAb/Phospho-NF-kappaB+p65+(Ser536)+Rabbit+mAb/pm40640139-324-11-12
    Average 99 stars, based on 9256 article reviews
    p nk κb - by Bioz Stars, 2026-09
    99/100 stars

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    Incubation:

    Article Title: Targeting modulation of the choroid plexus blood-CSF barrier and CSF hypersecretion via lipid nanoparticle-mediated co-delivery of siRNA and resveratrol
    Article Snippet: .. The membranes were then incubated overnight at 4 °C with primary antibodies: p-NK-κB (CST, 3033, 1:1000), NF-κB (CST,8242,1:1000), TNF-α (Abcam, ab307164,1:1000), p-SPAK (Sigma, 07-2273,1:1000), p-mTOR (Abcam, ab131538,1:2000), p-PI3K (Abcam, ab191606, 1:1000), p-AKT (Abcam, ab38449,1:1000), CCL2 (Abcam, ab7202,1:1000), IL-1β (Abcam,ab283818,1:1000), p-NKCC1 (Sigma,ABS1004, 1:1000). ..

    Article Title: Targeting modulation of the choroid plexus blood-CSF barrier and CSF hypersecretion via lipid nanoparticle-mediated co-delivery of siRNA and resveratrol.
    Article Snippet: .. The membranes were then incubated overnight at 4 °Cwith primary antibodies: p-NK-κB (CST, 3033, 1:1000), NF-κB (CST,8242,1:1000), TNF-α (Abcam, ab307164,1:1000), p-SPAK (Sigma, 07-2273,1:1000), p-mTOR (Abcam, ab131538,1:2000), p-PI3K (Abcam, ab191606, 1:1000), p-AKT (Abcam, ab38449,1:1000), CCL2 (Abcam, ab7202,1:1000), IL-1β (Abcam,ab283818,1:1000), p-NKCC1 (Sigma,ABS1004, 1:1000). ..



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    Inhibition of p-STAT3 or activation of p-NF-κB <t>p65</t> reverses the Shp2-/- induced cells migration. A. CT26 cells were in-direct co-cultured with BMDMs for 24 hours before proteins were extracted from BMDMs for western blot analysis to detect the p-NF-κB p65, NF-κB p65 and p-STAT3 expressions. Shp2MWT or Shp2MKO BMDMs were either pre-treated with AG490 at 100 µM for 1 hour or PMA at 20 ng/ml for 6 hours before co-cultured with CT26 cells. B. 24 hours later, proteins were extracted, and western blotting was performed to determine the p-NF-κB p65, NF-κB p65 and p-STAT3 expressions in BMDMs and the MMP2, MMP3 and MMP9 expressions in CT26 cells. Results were representative of 3 independent experiments. C. At 36 hours later, migration capacity was determined by Transwell migration assay. Results were obtained from 3 independent experiments and representative images are presented.
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    Inhibition of p-STAT3 or activation of p-NF-κB <t>p65</t> reverses the Shp2-/- induced cells migration. A. CT26 cells were in-direct co-cultured with BMDMs for 24 hours before proteins were extracted from BMDMs for western blot analysis to detect the p-NF-κB p65, NF-κB p65 and p-STAT3 expressions. Shp2MWT or Shp2MKO BMDMs were either pre-treated with AG490 at 100 µM for 1 hour or PMA at 20 ng/ml for 6 hours before co-cultured with CT26 cells. B. 24 hours later, proteins were extracted, and western blotting was performed to determine the p-NF-κB p65, NF-κB p65 and p-STAT3 expressions in BMDMs and the MMP2, MMP3 and MMP9 expressions in CT26 cells. Results were representative of 3 independent experiments. C. At 36 hours later, migration capacity was determined by Transwell migration assay. Results were obtained from 3 independent experiments and representative images are presented.
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    Inhibition of p-STAT3 or activation of p-NF-κB p65 reverses the Shp2-/- induced cells migration. A. CT26 cells were in-direct co-cultured with BMDMs for 24 hours before proteins were extracted from BMDMs for western blot analysis to detect the p-NF-κB p65, NF-κB p65 and p-STAT3 expressions. Shp2MWT or Shp2MKO BMDMs were either pre-treated with AG490 at 100 µM for 1 hour or PMA at 20 ng/ml for 6 hours before co-cultured with CT26 cells. B. 24 hours later, proteins were extracted, and western blotting was performed to determine the p-NF-κB p65, NF-κB p65 and p-STAT3 expressions in BMDMs and the MMP2, MMP3 and MMP9 expressions in CT26 cells. Results were representative of 3 independent experiments. C. At 36 hours later, migration capacity was determined by Transwell migration assay. Results were obtained from 3 independent experiments and representative images are presented.

    Journal: American Journal of Cancer Research

    Article Title: Tumor-associated macrophages (TAMs) depend on Shp2 for their anti-tumor roles in colorectal cancer

    doi:

    Figure Lengend Snippet: Inhibition of p-STAT3 or activation of p-NF-κB p65 reverses the Shp2-/- induced cells migration. A. CT26 cells were in-direct co-cultured with BMDMs for 24 hours before proteins were extracted from BMDMs for western blot analysis to detect the p-NF-κB p65, NF-κB p65 and p-STAT3 expressions. Shp2MWT or Shp2MKO BMDMs were either pre-treated with AG490 at 100 µM for 1 hour or PMA at 20 ng/ml for 6 hours before co-cultured with CT26 cells. B. 24 hours later, proteins were extracted, and western blotting was performed to determine the p-NF-κB p65, NF-κB p65 and p-STAT3 expressions in BMDMs and the MMP2, MMP3 and MMP9 expressions in CT26 cells. Results were representative of 3 independent experiments. C. At 36 hours later, migration capacity was determined by Transwell migration assay. Results were obtained from 3 independent experiments and representative images are presented.

    Article Snippet: After blocking with 5% non-fat dry milk, membranes were incubated overnight at 4°C with the following primary antibodies: anti-Shp2 (sc-7384; Santa Cruz), anti-GAPDH (#60004-1-I g) anti-MMP2 (#10373-2-AP), anti-MMP3 (#17873-1-AP), anti-MMP9 (#10375-2-AP; all from Proteintech, Wuhan, China), anti-p-NK-κB p65 (#3039), anti-NK-κB p65 (#8242), anti-p-STAT3 (#9145), anti-p-ERK (#4370), anti-ERK (#4695), anti-p-p38 (#9211), anti-p38 (#9212), anti-p-JNK (#4668), anti-JNK (#9252), anti-p-STAT1 (#9167), anti-STAT1 (#9172), anti-p-AKT (#4060) and anti-AKT (#4691; all from CST).

    Techniques: Inhibition, Activation Assay, Migration, Cell Culture, Western Blot, Transwell Migration Assay